NEURAL10 GENOMICS

The emergence of next-generation sequencing (NGS) has marked a before and after in the advancement of scientific research in Biology and Health Sciences, as well as in some diagnostic processes and clinical screening . More recently, single cell sequencing technologies, which represent an application of NGS techniques, have made it possible to study biological processes with an unprecedented level of detail. Through these techniques, it is possible to obtain a large amount of information related to various cellular processes, typically gene transcription and expression, which are considered "big data".



In order to acquire a biological meaning, this type of data requires an intense process of filtering, analysis and interpretation; which has given way to the creation of a new branch of science called bioinformatics. Bioinformatics is an interdisciplinary science that combines different mathematical, statistical, and computational methods for the analysis of complex biological data. In other words, bioinformatics is the meeting point of programming and mathematics with biology to give biological meaning to data from experiments, and thus test theories and hypotheses.
Today, bioinformatics has become an essential tool for the processing and analysis of data from NGS, both in the research context and in the applied clinical context. These analyzes are usually guided and interpreted by expert personnel in the biological sciences of study, thus allowing important scientific advances in these areas.
In recent years, this branch of science has advanced by leaps and bounds, where various methods that were previously specific to computational systems have been adapted and/or implemented to analyze large amounts of biological data with a wide variety of objectives. However, bioinformatics is not an easy science to learn, and if it is not implemented correctly, it can lead to erroneous conclusions affecting the final results of the experiment. In addition, the large number of tools available (there are currently more than 1,000 public bioinformatics tools (TODO: cites lazzapi website) codified on various computational platforms) and the complexity and specificity of the analyzes make it difficult or even impossible to implement this type of analysis. in research laboratories.

The interdisciplinary team of scientists at Neural10 presents a solution to this problem by offering the following services:

  • Personalized training. We offer training to laboratory personnel adapted to the needs of their research/clinical project so that learning is as clear and useful as possible based on the objectives of each research group. Because we are an interdisciplinary group of researchers, we can understand your needs from different points of view and thus structure a training program according to the level of experience and training profile of your laboratory staff.


  • Analysis of data. One of the most important factors in research is the time it takes to obtain results. As a group of experts in bioinformatics, we offer personalized and efficient data analysis services. With this, your work team can focus on other activities in your field of expertise while we process and analyze your data in order to obtain quality results in the shortest time possible, which can be published directly, validated experimentally, or involve the new generation of hypotheses and possibly the redirection of the research itself.

LIST OF SEVICES OFFERED

RNA

Single Cell



Basic



  • Mapping sequencing data to a reference genome and quantifying gene expression.
  • Quality control of samples and genes.
  • Data normalization and preprocessing.
  • Grouping and dimensionality reduction of data.
  • Identification of populations with markers at a basic level.
  • Analysis of “pathways” and “gene ontologies” (GO) by population.
  • Integration of samples and analysis of genetic differences by groups at the basic level.


Advanced



  • Demultiplexing of samples processed with “hashtag antibodies”.
  • Analysis and identification of subpopulations with markers at advanced levels.
  • Analysis of “pathways” and GO by subpopulations.
  • Pseudo-time and differentiation lineages.
  • RNA velocity analysis (if applicable).
  • Integrated analysis of gene expression and other variables (eg repertoire or “VDJ sequencing”) in the same cells.

Bulk



Basic



  • Mapping sequencing data to a reference genome and quantifying gene expression.
  • Quality control of samples and genes.
  • Data normalization and preprocessing.
  • Comparison between groups.
  • Variable identification by groups


Advanced



  • Mapping sequencing data to a reference genome and quantifying gene expression.
  • Analysis of “pathways” and GO by population.

DNA

ChiP-seq

ATAQ-seq



All our services include the deliverable of the analysis code, up to 5 high-quality figures to be included in your main results, and up to 8 additional figures. The delivery time for the results of our basic services is approximately 30 business days. During this time, four meetings with the Neural10 Genomics research team will take place as follows:



  1. Start of the project. This meeting, held at the beginning of the project, has the objective of discussing the origin of the data to propose a list of preliminary objectives.


  2. Preliminary results. The Neural10 team is committed to having preliminary results within 7 business days. These results will be sent to your research group for analysis and discussion in preparation for a second meeting with the Neural10 research group, usually 10 days after the start of the project. In said meeting, the results will be evaluated based on the preliminary objectives to decide if the research course is valid, or if necessary, rethink the objectives of the analysis.


  3. Intermediate results. After the validation of the preliminary results, the Neural10 team will work on refining the preliminary results, or on obtaining results based on the new research objectives. Approximately 20 business days after the start of the project, a third meeting will be held to present the intermediate results of the project. These results are a preliminary of the final results, where the results obtained for the design of the final figures of the project will be evaluated.


  4. Final results. At the fourth meeting, held approximately 30 business days from the start of the project, the final figures of the project and the analyzes carried out will be delivered